Aktivitas Antioksidan Ekstrak Etanol Ulva sp dan Gracilaria sp dari Pantai Sayang Heulang

Windy Widowaty; Sri Maryam; Nurul Novianti; Lita Mulyani

doi:10.17977/um0260v7i12023p023

Authors

Windy Widowaty Program Studi Teknologi Pangan, Fakultas Teknologi Industri Pertanian, Universitas Al Ghifari, Indonesia
Sri Maryam Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Al Ghifari, Indonesia.
Nurul Novianti Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Al Ghifari, Indonesia.
Lita Mulyani Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Al Ghifari, Indonesia.

DOI:

https://doi.org/10.17977/um0260v7i12023p023

Keywords:

Ulva sp, Gracilaria sp, FRAP

Abstract

Ulva sp and Gracilaria sp are found on the coast of Sayang Heulang beach and have potential as natural
antioxidants. This study aims to determine the antioxidant capacity of the two types of seaweed using the FRAP (Ferric Reducing Antioxidant Power) method. Ulva sp and Gracilaria sp were taken from Sayang Heulang
Beach in March 2022. Each type of seaweed extracted using 70% ethanol for 3x24 hours.The extract then
concentrated using a rotary evaporator for further analysis. The results of phytochemical screening showed that the ethanolic extract of Ulva sp was positive for flavonoids, tannins, saponins, and alkaloids, while the Gracilaria sp extract was positive for alkaloids, flavonoid tannins, steroids, and saponins. The antioxidant capasity of Ulva sp and Gracilaria sp ethanol extracts carried using the FRAP method with ascorbic acid used as standard. Each extract was measured using a UV-Vis spectrophotometer with a wavelength of 694 nm. The measurement results were expressed as mg equivalent of ascorbic acid/gram extract (mg AAE/gr sample). The analysis showed that the ethanolic extracts of Ulva sp and Gracilaria sp had antioxidant capacity values of 1.8 mg AAE/gr sample and 5.50 mg AAE/gr sample, respectively.