Identifikasi Fitosterol dengan Kromatografi Gas – Spektrometer Massa pada Ekstrak Kloroform Biji Buah Atung (Parinarium Glaberimum Hassk) Asal Kabupaten Seram Bagian Barat, Maluku

Candra Yulius Tahya; Elen Tiwery; Mega Gracelia Monaten; Tryani K. Junita Lumbantoruan

doi:10.17977/um0260v4i12020p014

Authors

Candra Yulius Tahya Program Studi Pendidikan Kimia, Fakultas Ilmu Pendidikan, Universitas Pelita Harapan, Indonesia.
Elen Tiwery SMP Negeri 1 Kairatu Barat, Maluku, Indonesia
Mega Gracelia Monaten Fakultas Kedokteran, Univeritas Pattimura, Indonesia
Tryani K. Junita Lumbantoruan Fakultas Pertanian, Universitas Pattimura, Indonesia

DOI:

https://doi.org/10.17977/um0260v4i12020p014

Keywords:

Atung, Chloroform Extract, GC-MS, Phytosterol, γ-Sitosterol

Abstract

One of the natural potentials that can be used as traditional medicine and natural preservatives is the
seeds of Atung which can be found in Ambon, Seram and Lease Islands. The purpose of this study was to
identify bioactive phytosterols in the chloroform extract of Atung fruit seeds. Five main compounds have been
identified in the chloroform extract of Atung fruit seeds (Parinarium glaberimum Hassk) which are linoleic
acid (8.88%); stearic acid (8.23%); stigmasta-5,22-dien-3-ol,(3β,22E) or stigmasterol (10.37%); stigmast-5-
en-3-ol,(3β) or β-sitosterol (19.59%); and stigmast-5-en-3-ol, (3β, 24S) or γ-sitosterol ( 33.74%) through gas
chromatography-mass spectroscopy by analyzing the similarity of molecular weight and positive ion
mass/charge fragments and based on the comparison of the GC-MS retention time of phytosterols. This study
is the first to report the presence of stigmasterol, β-sitosterol and γ-sitosterol in the chloroform extract of
Atung fruit seeds (Parinarium glaberimum Hassk). γ -Sitosterol, the epimer of β-sitosterol, has the greatest
abundance in the chloroform extract of Atung fruit seeds.