Sintesis Wax Ester dari Asam Lemak Stearat secara Enzimatis Sebagai Bahan Baku Kosmetik

Abstract

The purpose of this study is to synthesize wax ester from fatty acid stearic with oleil alcohol, determine the
optimum conditions and to find out what percentage of wax ester is produced. Wax ester synthesis is
carried out enzymatically. Qualitative wax ester analysis using TLC and FT-IR, quantitatively by acid-base
titration and determination of optimum conditions using a variation of time, temperature, number of
enzymes. Based on the results of the TLC analysis obtained the Rf value of 0.14, FT-IR spectrum of stearic
fatty acids, oleil alcohol and wax ester can be concluded that wax ester can be synthesized from fatty acid
stearic with oleil alcohol enzymatically. The optimum conditions for wax ester synthesis from stearic fatty
acid for 2 hours, at 40 ° C, with a lipase amount of 0.15 grams, in the ratio of stearic acid: oleil alcohol =
1: 3 and with n-hexane as the best solvent. The percentage of conversion obtained is 94%.