Inhibisi Xantin Oksidase oleh Ekstrak Daun Salam (Syzygium Polyanthum)

Abstract

Xhantine oxidase is an enzyme that plays a role in the formation of uric acid. Xanthine oxidase catalyzes
hypoxanthine into xantin and then becomes uric acid. Bay leaf plants can lower blood uric acid level
because it contains secondary metabolites such as flavonoid, tannin, and essential oil. This reseach
aimed are to find out xhantine oxidase inhibition by bay leaf extract. This reseach use experimental
research method which done in pharmacognosi of pharmacy of Putra Indonesia Malang. The reseach
include extraction, isolation of xanthine oxidase enzyme, enzyme activity test and enzyme inhibition test.
Testing of enzyme activity of xanthine oxidase inhibition using spectrophotometric at λ290 nm. The
result showes that in variant concentrations of 5 ppm, 10 ppm, 20 ppm, 50 ppm dan 100 ppm were
produced inhibitions is %, 0%, 100%, 100%, 0%. Allopurinol is used as a positive control with the same
concentration, the % inhibition is 100%, 0%, 0%, 0%, dan 100%. Based on the result, the bay leaf
extract with a concentration of 5 ppm has 100% inhibition equivalent to allopurinol 5 ppm.
Concentration 100 ppm of bay leaf extract not show inhibitory activity.