Aktivitas Antibakteri Metabolit Sekunder dari Ekstrak Metanol Biji Alpukat (Persea americana Mill)

Abstract

An antibacterial activity of secondary metabolite has been isolated from methanol extract of avocado seed (Parsea americana Mill). The isolation process has started with extracting using methanol, then fractioning and purifying by chromatography technique such as vacuum liquid chromatography and gravitation column chromatography. The white solid of K-1 component has been isolated from this process. The melting point of K-1 component was 67-70˚C and dissolved in chloroform, ethyl acetate, and ethanol. K-1 component has λmaks at 219,0 nm (π→π* transition) and 344,0 nm (n→π* transition). Based on IR spectrum interpretation, this component contained –OH group, -C=O group of ester, -CH bond, and C=C bond. K-1 component was predicted as saponin group. The antibacterial activity assay against Escherichia coli and Staphylococcus aureus indicated that methanol extract of avocado seed and K-1 component showed antibacterial activity toward both bacteria, so that this component has future chances for traditional medicine development.