Aktivitas Antioksidan Ekstrak Biji Buah Areca vestiaria Giseke dan Fraksinya dengan Metode DPPH (2,2-diphenyl-1-picrylhydrazyl)

Abstract

Antioxidant widely used to prevent degeneratif desease and anti aging. The seed of Pinang yaki fruits
contain some compounds which have antioxidant activity. The purposes of this research were to know the antioxidant activity of ethanolic extracts and fractions of the seed of pinang yaki fruits and the IC50 value of its antioxidant activity. The samples of the seed were prepared separately by soxhlet extraction method with ethanol 96% as the solvent. Then fractionation process used three different solvents are n-hexane, ethyl acetate, and ethanol-water. Futhermore, the calculation of yield, phytochemical screening, and test of antioxidant activity using DPPH method. Yield calculation result showed ethanolic extract, fraction of nhexane, fraction of ethyl acetate, fraction of ethanol-water 17,1%, 10,8%, 18,2%, 52,8%. Phytochemical screening of Flavonoids ethanolic extract, fraction of ethyl acetate, fraction of ethanol-water showed positive contains Flavonoid. IC50 value of ethanolic extract, fraction of n-hexane, fraction of ethyl acetate, fraction of ethanol-water 94,5 µg/mL, 445,7 µg/mL, 44,7 µg/mL, 91,4 µg/mL. The conclusion of this research is extract of ethanol and the fraction of seed of pinang yaki fruits has the antioxidant activity. Fraction of ethyl acetate has the higher antioxidant activity 44,7 µg/mL.